Titlebook: RNA Chaperones; Methods and Protocol Tilman Heise Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 RNA biology.

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Detection of MicroRNAs Released from Argonautes,: release of miRNA from associated AGO proteins. In this chapter, we describe in detail a methodology derived from biochemical approaches, which can be used to quantify the release of any given miRNA from AGOs.

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Delivering Molecular Beacons via an Electroporation-Based Approach Enables Live-Cell Imaging of Sin this chapter, we describe in detail microporation steps for MB delivery that we have utilized over the past several years, followed by examples demonstrating successful MB-based imaging of specific RNA transcripts and genomic loci at the single-molecule level.

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Isolation and Analysis of Bacterial Ribosomes Through Sucrose Gradient Ultracentrifugation, gradient allows for the recovery of 70S ribosomes and its subunits enabling a wide range of downstream applications. This protocol can be easily adapted to ribosome-related studies in other species or for separating other macromolecular complexes.

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Hyeong Jin Kim,Steven Chaulk,David Arthur,Ross A. Edwards,J. N. Mark Glover

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Subrata Panja,Ewelina M. Małecka,Andrew Santiago-Frangos,Sarah A. Woodson

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