Titlebook: RNA Chaperones; Methods and Protocol Tilman Heise Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 RNA biology.

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Site-Specific Dual-Color Labeling of Long RNAs,d with reporting entities via bioorthogonal chemistry. This method is particularly valuable for, but not limited to, single-molecule fluorescence applications. We demonstrate the method on an RNA construct of 275 nucleotides, the . riboswitch of ..

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1064-3745 experts.This book provides a wide spectrum of methods to study RNA chaperones .in vitro., at the single molecule level, and protocols useful for cell-based assays. Beginning with a section on a number of bacterial proteins for study, the volume also explores proteins from eukaryotic cells and how t

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Quantitative Analysis of RNA Chaperone Activity by Native Gel Electrophoresis and Fluorescence Specinal RNA duplex. The . protein Hfq facilitates interactions between small noncoding RNAs and their target mRNAs. The chaperone and RNA annealing activity of Hfq and other RNA chaperones can be evaluated by determining the kinetics of RNA base pairing in the presence and absence of the protein. This

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