找回密码
 To register

QQ登录

只需一步,快速开始

扫一扫,访问微社区

Titlebook: RNA; Methods and Protocol Henrik Nielsen Book 2011 Springer Science+Business Media, LLC 2011 Bioinformatics.Mammalian genome.Messenger RNAs

[复制链接]
查看: 43981|回复: 56
发表于 2025-3-21 16:16:14 | 显示全部楼层 |阅读模式
书目名称RNA
副标题Methods and Protocol
编辑Henrik Nielsen
视频video
概述Features samples for subsequent analysis by high-throughput technologies, such as deep sequencing or microarray analysis -Contains thorough, detailed methods and protocols easily implemented by novice
丛书名称Methods in Molecular Biology
图书封面Titlebook: RNA; Methods and Protocol Henrik Nielsen Book 2011 Springer Science+Business Media, LLC 2011 Bioinformatics.Mammalian genome.Messenger RNAs
描述Recent insight into the transcripts generated from the mammalian genome (i.e. the transcriptome) has revealed that transcription is a far more complex phenomenon than previously thought.In RNA: Methods and Protocols, expert researchers provide the procedures and methods used to describe the structure of messenger RNAs and non-coding RNAs that are transcribed by RNA polymerase II as the immediate gene products in mammalian cells.Focused on the structure of the RNA products of “gene X” and the mapping of proteins associated with these RNAs, the volume presents appropriate information for non-specialists in RNA biology. Written in the highly successful Methods in Molecular Biology™ series format, many chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, RNA: Methods and Protocols views the transcriptional landscape with an appreciation for the role that proteins play in the processing and interpretation of genetic information in an attempt to further our crucial knowledge of the many produ
出版日期Book 2011
关键词Bioinformatics; Mammalian genome; Messenger RNAs; Microarray analysis; Non-coding RNAs; Protein mapping; R
版次1
doihttps://doi.org/10.1007/978-1-59745-248-9
isbn_softcover978-1-4939-5643-2
isbn_ebook978-1-59745-248-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2011
The information of publication is updating

书目名称RNA影响因子(影响力)




书目名称RNA影响因子(影响力)学科排名




书目名称RNA网络公开度




书目名称RNA网络公开度学科排名




书目名称RNA被引频次




书目名称RNA被引频次学科排名




书目名称RNA年度引用




书目名称RNA年度引用学科排名




书目名称RNA读者反馈




书目名称RNA读者反馈学科排名




单选投票, 共有 0 人参与投票
 

0票 0%

Perfect with Aesthetics

 

0票 0%

Better Implies Difficulty

 

0票 0%

Good and Satisfactory

 

0票 0%

Adverse Performance

 

0票 0%

Disdainful Garbage

您所在的用户组没有投票权限
发表于 2025-3-21 21:29:01 | 显示全部楼层
发表于 2025-3-22 04:27:48 | 显示全部楼层
Working with RNAk rules have to be implemented to maintain the integrity of the RNA. Alkaline pH, high temperatures, and heavy metal ions should be avoided when possible and ribonucleases kept in check. The chapter outlines the specific precautions recommended for work with RNA and describes some of the modificatio
发表于 2025-3-22 07:57:22 | 显示全部楼层
Synthesis of RNA by In Vitro Transcriptionto those of several kilobases in μg to mg quantities. It is based on the engineering of a template that includes a bacteriophage promoter sequence (e.g. from the T7 coliphage) upstream of the sequence of interest followed by transcription using the corresponding RNA polymerase. In vitro transcripts
发表于 2025-3-22 11:08:29 | 显示全部楼层
Efficient Poly(A)+ RNA Selection Using LNA Oligo(T) Capturepoly(A). RNA directly from guanidinium thiocyanate (GuSCN)-containing cell or tissue extract by combining the design of biotinylated LNA oligo(T) capture probes with subsequent immobilization of the captured poly(A). RNA onto streptavidin-coated magnetic particles. In contrast to DNA oligo-dT and po
发表于 2025-3-22 15:39:14 | 显示全部楼层
发表于 2025-3-22 20:52:47 | 显示全部楼层
发表于 2025-3-22 21:50:23 | 显示全部楼层
发表于 2025-3-23 01:47:22 | 显示全部楼层
Rapid Amplification of cDNA Ends (RACE)ly known. Starting with an mRNA mixture, gene-specific primers generated from the known regions of the gene and non-specific anchors, full-length sequences can be identified in as little as 3 days. RACE can also be used to identify alternative transcripts of a gene when the partial or complete seque
发表于 2025-3-23 07:45:34 | 显示全部楼层
Fractionation of mRNA Based on the Length of the Poly(A) Taile mRNAs according to the length of the poly(A) tail. Poly(A) fractionation can be used to detect small changes in poly(A) tail length or to prepare samples for microarray analysis. RNA or crude lysate is mixed with biotinylated oligo(dT), which is then bound to paramagnetic streptavidin beads. Oligo
 关于派博传思  派博传思旗下网站  友情链接
派博传思介绍 公司地理位置 论文服务流程 影响因子官网 SITEMAP 大讲堂 北京大学 Oxford Uni. Harvard Uni.
发展历史沿革 期刊点评 投稿经验总结 SCIENCEGARD IMPACTFACTOR 派博系数 清华大学 Yale Uni. Stanford Uni.
|Archiver|手机版|小黑屋| 派博传思国际 ( 京公网安备110108008328) GMT+8, 2025-5-2 12:55
Copyright © 2001-2015 派博传思   京公网安备110108008328 版权所有 All rights reserved
快速回复 返回顶部 返回列表