Titlebook: R-Loops; Methods and Protocol Andrés Aguilera,Alexey Ruzov Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive licen

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1064-3745 hooting and avoiding known pitfalls. .Authoritative and practical, .R-Loops: Methods and Protocols. serves as an ideal resource for those working on R-loop ho978-1-0716-2479-1978-1-0716-2477-7Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Direct R-Loop Visualization on Genomic DNA by Native Automated Electron Microscopy,e preparation procedures that protect R-loop stability allows for direct visualization of R-loop structures on genomic DNA, independently from probes. Combining single-molecule information and DNA content assessment, this approach provides direct estimations of R-loop frequency, size, and burden on

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Detection of R-Loop Structures by Immunofluorescence Using the S9.6 Monoclonal Antibody,me-wide, whereas Immunofluorescence is performed to visualize R-loops in single cells. While mapping R-loops genome-wide is very important for identifying and studying their location-specific role, microscopy offers the advantage of spatial information and the ability to quantify them on a single ce

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Sónia Silva,Cristina Guillén-Mendoza,Andrés Aguilera

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1064-3745 expertsThis detailed book compiles a series of laboratory protocols covering the most important aspects of R-loop biology. Beginning with a range of methods allowing for the detection of DNA-RNA hybrids, as well as their purification and visualization by electron microscopy, the volume continues wi

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Direct R-Loop Visualization on Genomic DNA by Native Automated Electron Microscopy,abundance and/or size has been suggested to interfere with the DNA replication process, contributing to genome instability. Most available approaches to monitor R-loops are based on antibodies/enzymes that cannot effectively distinguish R-loops from DNA–RNA hybrids and assess R-loop size and frequen