| 书目名称 | R-Loops |
| 副标题 | Methods and Protocol |
| 编辑 | Andrés Aguilera,Alexey Ruzov |
| 视频video | |
| 概述 | Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | This detailed book compiles a series of laboratory protocols covering the most important aspects of R-loop biology. Beginning with a range of methods allowing for the detection of DNA-RNA hybrids, as well as their purification and visualization by electron microscopy, the volume continues with methods based on the use of RNase H-derived tools to detect DNA-RNA hybrids .in vitro. and .in vivo.. Several protocols permit studying non-canonical RNA nucleotides in the R-loop context, as well as a number of specific protocols devoted to the investigation of R-loop topology and their functional roles in the biology of mitochondria and telomeres. Finally, a large block of chapters is dedicated to different methods allowing genome-wide mapping of DNA-RNA hybrids in various organisms. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .R-Loops: Methods and Protocols. serves as an ideal resource for those working on R-loop ho |
| 出版日期 | Book 2022 |
| 关键词 | DNA-RNA hybrids; Genomic stress and instability; Genome dynamics; Electron microscopy; Topology; Genome-w |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-0716-2477-7 |
| isbn_softcover | 978-1-0716-2479-1 |
| isbn_ebook | 978-1-0716-2477-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
发表于 2025-3-21 18:24:24
