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Titlebook: Quantitative Methods in Proteomics; Katrin Marcus,Martin Eisenacher,Barbara Sitek Book 2021Latest edition Springer Science+Business Media,

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发表于 2025-3-21 19:19:35 | 显示全部楼层 |阅读模式
书目名称Quantitative Methods in Proteomics
编辑Katrin Marcus,Martin Eisenacher,Barbara Sitek
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Quantitative Methods in Proteomics;  Katrin Marcus,Martin Eisenacher,Barbara Sitek Book 2021Latest edition Springer Science+Business Media,
描述.This second edition provides new and updated methods on the principles underlying modern protein analysis, from statistical issues to gel-based and mass spectrometry-based applications. Chapters detail protein quantification as basis for realisation of quantitative studies, gel-based and mass spectrometry-based quantification techniques, TMT, IPTL, PRM, MALDI Imaging, SILAC, PTM analysis, DIA, cross-linking, and the up-to-date topics of software and data analysis. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls... Authoritative and cutting-edge,  .Quantitative Methods in Proteomics, Second Edition .aims to provide comprehensive and competent overview in the important and still growing field of quantitative proteomics..
出版日期Book 2021Latest edition
关键词Westernblotting; 2D-DIGE; iTRAQ; Plasma analysis,; Quantitative Secretomics; Proteomics
版次2
doihttps://doi.org/10.1007/978-1-0716-1024-4
isbn_softcover978-1-0716-1026-8
isbn_ebook978-1-0716-1024-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

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Quantitative Methods in Proteomics978-1-0716-1024-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
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发表于 2025-3-22 12:13:48 | 显示全部楼层
https://doi.org/10.1007/978-1-0716-1024-4Westernblotting; 2D-DIGE; iTRAQ; Plasma analysis,; Quantitative Secretomics; Proteomics
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Important Issues in Planning a Proteomics Experiment : Statistical Considerations of Quantitative Pimes neglected part in detecting differential proteins is the statistical analysis. Data from proteomics experiments are usually high-dimensional and hence require profound statistical methods. It is especially important to already correctly design a proteomic experiment before it is conducted in th
发表于 2025-3-22 21:40:35 | 显示全部楼层
Good Old-Fashioned Protein Concentration Determination by Amino Acid Analysis,still this relevance within proteomics for multiple reasons especially in the quantitative protein analysis. Amino acid analysis enables indirectly both the protein and peptide concentration determination which are essential for using the same amounts for comparative quantitative experiments. Moreov
发表于 2025-3-23 01:56:08 | 显示全部楼层
,Protein Quantification Using the “Rapid Western Blot” Approach,tion of a target protein based on the use of specific antibodies. However, the whole procedure is often very time-consuming. Nevertheless, with the development of fast blotting systems and further development of immunostaining methods, a reduction of the processing time can be achieved. Major challe
发表于 2025-3-23 09:01:02 | 显示全部楼层
The Whereabouts of 2D Gels in Quantitative Proteomics,teomics, its unique features make it a still highly valuable tool, especially when multiple quantitative comparisons of samples must be made, and even for large samples series. However, quantitative proteomics using two-dimensional gels is critically dependent on the performances of the protein dete
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