Titlebook: Quantitative Methods in Proteomics; Katrin Marcus,Martin Eisenacher,Barbara Sitek Book 2021Latest edition Springer Science+Business Media,

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Proteome Analysis with Classical 2D-PAGE,on, proteins are separated by their isoelectric point, a technique known as isoelectric focusing (IEF). There are two important variants of IEF, which are carrier-ampholine (CA)-based IEF and immobilized pH-gradient (IPG)-based IEF. In the second dimension, proteins are further separated by their el

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Quantitative Approach Using Matrix-Assisted Laser Desorption/ Ionization Time-of-Flight (MALDI-ToF)latforms are typically employed, as they provide highly reproducible outputs for batch processing of multiple samples. Quantitation using matrix-assisted laser desorption/ionization (MALDI) time-of-flight (ToF) mass spectrometry, while less commonly adopted, offers the ability to monitor analytes at

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