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Titlebook: Psychoneuroimmunology; Methods and Protocol Qing Yan Book 2025Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi

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Hair and Saliva Endocannabinoid and Steroid Hormone Analysis by Liquid Chromatography Paired with Tandem Mass Spectrometrynoids in hair and saliva samples is possible using liquid chromatography paired with tandem mass spectrometry (LC-MS/MS). This chapter describes two simple sample preparation methods that can be used to prepare hair and saliva samples for analysis using LC-MS/MS. Our LC-MS/MS method can be applied t
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Mouse Testing Methods in Psychoneuroimmunology: Measuring Behavioral Responsesor is a consequence of such interactions and manifests from a complex interweave of factors including immune-to-neural and neural-to-immune communication. Often the signaling molecules involved during a particular episode of neuroimmune activation are not known, but behavioral response provides evid
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Methods for Modeling Early Life Stress in Rodentsrauma has expanded over recent decades. Rodent models are a cornerstone of the ELS literature with many studies utilizing paradigms based on early life separation/deprivation protocols and manipulating the cage environment. However, no animal model is perfect. In particular, the lack of standardizat
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The MRL Model: A Valuable Tool in Studies of Autoimmunity-Brain Interactionsvidence points to causal relationships between immune factors, neurodegeneration, and neuropsychiatric manifestations. By documenting autoimmunity-associated neuronal degeneration and cytotoxicity of the cerebrospinal fluid from disease-affected subjects, the murine MRL model has shown high validity
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A Computational Pipeline for Identifying Gene Regulatory Networks: A Case Study of Response to Exerciseranging from metabolic rate to the response to a drug or other intervention. The data required to accurately identify these control networks remains very cost and labor intensive typically leading to relatively sparse time course data that is largely incompatible with conventional data-driven model
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Microglial and Macrophage Plasticity and Regional Cerebral Blood Flow in the Prenatal Brain and Gut Under Vagus Nerve Stimulationbout the connection between the vagus nerve and the brain’s regional circulatory control. In this chapter, we present a methodology for studying the impact of vagus nerve signaling on these connections in the developing fetus using the sheep model for human fetal physiology. First, we present the pr
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Perinatal Psychoneuroimmunology of Prenatal Stress and Its Effects on Fetal and Postnatal Brain Developmentchanisms of such fetal programming. A pregnant sheep model of maternal stress offers the unique advantages of chronic in utero monitoring and manipulation. This chapter presents the techniques used to model single and multigenerational stress exposures and their pleiotropic effects on the offspring.
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The Integrative Single-Case Design as a Biosemiotic-Systemic Research Tool in Psychoneuroimmunologying these complex PNI relationships under as ecologically valid as possible conditions (“life as it is lived”) necessitates a paradigm change in research. This shift places factors such as “time” and “subjective meaning” of personal experiences at the center of the research methodology. For this pur
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