Titlebook: Optical Tweezers; Methods and Protocol Arne Gennerich Book 2017 Springer Science+Business Media New York 2017 optical tweezers setups.singl

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Marian Baclayon,Svenja-Marei Kalisch,Ed Hendel,Liedewij Laan,Julien Husson,E. Laura Munteanu,Marileerige Geschichte von Auseinandersetzungen vorausgegangen, die zu einer klaren Dominanz der versorgungsorientierten Psychiatrie in der Region in dieser Zeitspanne geführt hatten. 1827 hatte das Innenministerium von Baden Christian Friedrich W. Roller (1802–1878) beauftragt, an der Universität Heidelbe

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A Surface-Coupled Optical Trap with 1-bp Precision via Active StabilizationNA is difficult, since a variety of noise sources degrade instrumental stability. In this chapter, we detail how to improve instrumental stability by (1) minimizing laser pointing, mode, polarization, and intensity noise using an acousto-optical-modulator mediated feedback loop and (2) minimizing sa

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Implementation and Tuning of an Optical Tweezers Force-Clamp Feedback Systemosition or tension applied to a single biomolecule constant. Here, we describe the implementation of the latter: an optical force-clamp setup that can be used to study the motion of processive molecular motors under a constant load. We describe the basics of a software-implemented proportional-integ

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Custom-Made Microspheres for Optical Tweezerslds, such as biology, material science, and physics. Often, small dielectric particles are trapped and used as probes, which for experimental convenience are mostly spherical and composed of silica or polystyrene. The optical properties of these materials together with the microsphere size determine

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Optical Torque Wrench Design and Calibrationard linear optical tweezers are employed. Here we describe in detail the construction, alignment, and calibration of the Optical Torque Wrench, a mode of function that can be added to linear optical tweezers to simultaneously apply and measure both force and torque on birefringent microscopic cylind

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Versatile Quadruple-Trap Optical Tweezers for Dual DNA Experimentsl trapping is an emerging method for optical manipulation and force spectroscopy that has found its primary use in studying dual DNA interactions, but is certainly not limited to DNA investigations. The key benefit of quadruple optical trapping is that two molecular strands can be manipulated indepe