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Titlebook: Optical Tweezers; Methods and Protocol Arne Gennerich Book 2017 Springer Science+Business Media New York 2017 optical tweezers setups.singl

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发表于 2025-3-21 17:10:32 | 显示全部楼层 |阅读模式
书目名称Optical Tweezers
副标题Methods and Protocol
编辑Arne Gennerich
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Optical Tweezers; Methods and Protocol Arne Gennerich Book 2017 Springer Science+Business Media New York 2017 optical tweezers setups.singl
描述.The aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation .in vitro. and in living cells. Chapters have been divided in three parts focusing on theory and practical design of optical tweezers, detailed protocols for performing force measurements on single DNA- and microtubule/actin-associated mechanoenzymes in isolation, and describing recent advances that have opened up quantitative force measurements in living cells. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .Optical Tweezers: Methods and Protocols. aims help to further expand the accessibility and use of optical traps by scientists of diverse disciplines. .
出版日期Book 2017
关键词optical tweezers setups; single-molecule fluorescence imaging; force measurements; quantitative force m
版次1
doihttps://doi.org/10.1007/978-1-4939-6421-5
isbn_softcover978-1-4939-8194-6
isbn_ebook978-1-4939-6421-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2017
The information of publication is updating

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发表于 2025-3-21 22:52:08 | 显示全部楼层
Exact Theory of Optical Tweezers and Its Application to Absolute Calibrationyleigh regime to the ray optics one, for different polarizations and trapping heights, including all commonly employed parameter domains. Protocols for implementing absolute calibration are given, explaining how to measure all required experimental parameters, and including a link to an applet for stiffness calculations.
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Introduction to Optical Tweezerstific achievements, and demonstrate why optical traps have become a powerful tool in the biological sciences. We introduce a typical optical setup, describe the basic theoretical concepts of how trapping forces arise, and present the quantitative position and force measurement techniques that are most widely used today.
发表于 2025-3-22 10:36:02 | 显示全部楼层
Optical Torque Wrench Design and Calibrationrientation, described by a periodic potential. As a consequence of the experimental control of the tilt of the periodic potential, the dynamical excitability of the system can be observed. Angular optical tweezers remain less widespread than their linear counterpart. We hope this technical guide can foster their development and new applications.
发表于 2025-3-22 16:31:46 | 显示全部楼层
Probing Single Helicase Dynamics on Long Nucleic Acids Through Fluorescence-Force Measurementof fluorescence-force spectroscopy instrumentation, calibration, and activity assays in detail for observing the biochemical activities of helicases in real time and revealing how mechanical forces are involved in protein–nucleic acid interaction. These single-molecule approaches are generally applicable to many other protein–nucleic acid systems.
发表于 2025-3-22 18:28:42 | 显示全部楼层
Book 2017sts, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation .in vitro. and in living cells. Chapters have been divided in three parts focusing on theory and practical design of optical tweezers, detailed pro
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发表于 2025-3-23 04:20:52 | 显示全部楼层
Implementation and Tuning of an Optical Tweezers Force-Clamp Feedback Systemliance of the involved molecules. To investigate a particular mechanical process, understanding the basics and limitations of the feedback system will be helpful for choosing the proper feedback hardware, for optimizing the system parameters, and for the design of the experiment.
发表于 2025-3-23 06:10:10 | 显示全部楼层
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