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Titlebook: Nucleic Acid Crystallography; Methods and Protocol Eric Ennifar Book 2016 Springer Science+Business Media New York 2016 DNA.Macromolecule P

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书目名称Nucleic Acid Crystallography
副标题Methods and Protocol
编辑Eric Ennifar
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Nucleic Acid Crystallography; Methods and Protocol Eric Ennifar Book 2016 Springer Science+Business Media New York 2016 DNA.Macromolecule P
描述.This volume includes comprehensive and up-to-date coverage of all nucleic-acid-specific steps used in X-ray crystallography, from macromolecule production to structure determination. Chapters dedicated to RNA preparation and crystallogenesis will be of interest to beginners, while chapters focused on data collection, phasing and refinement will be particularly useful to researchers with a higher level of expertise. Several functional case studies are also presented in the last part of the book. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and thorough, .Nucleic Acid Crystallography: Methods and Protocols. presents protocols that are aimed at both researchers and students who are interested in the structural biology of DNA or RNA, alone or in complex with proteins or ligands..
出版日期Book 2016
关键词DNA; Macromolecule Production; Nucleic Acid Crystallography; Nucleic Acid Purification; Nucleic Acid Syn
版次1
doihttps://doi.org/10.1007/978-1-4939-2763-0
isbn_softcover978-1-4939-4641-9
isbn_ebook978-1-4939-2763-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
The information of publication is updating

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Preparation of Short 5′-Triphosphorylated Oligoribonucleotides for Crystallographic and Biochemical ny RNAs form specific interactions with proteins often using short nucleotide sequences for protein recognition. Biochemical and structural studies of such RNA–protein complexes demand preparation of short RNAs. Although short RNAs can be synthesized chemically, certain proteins require monophosphat
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