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Titlebook: Nucleic Acid Crystallography; Methods and Protocol Eric Ennifar Book 2016 Springer Science+Business Media New York 2016 DNA.Macromolecule P

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Nikita Vasilyev,Alexander Serganov life and to indicate what the future holds in terms of pig welfare. The text is aimed at researchers and teachers working in veterinary and animal science together with those working in the pig industry and for governmental and non-governmental animal welfare organizations..978-90-481-8024-0978-1-4020-8909-1Series ISSN 1572-7408
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Clément Dégut,Alexandre Monod,Franck Brachet,Thibaut Crépin,Carine Tisnéuture research. The meeting was a fruitful first step in bringing together different opinions in order to arrive at a universally acceptable common solution for modern pig production. W. Sybesma SESSION I BEHAVIOURAL AND PHYSIOLOGICAL NEEDS OF PIGS IN GENERAL I. BASIC NEEDS Chairman: J.P. Signoret BEHAVIOURAL978-94-011-9574-4
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Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction DataAD or MAD phasing. In many cases, suitable derivatives can be obtained by soaking the hexammine into RNA crystals that have not been engineered. Considerations for using this method and example protocols are presented.
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Polyacrylamide Gel Electrophoresis for Purification of Large Amounts of RNA by changing the percentage and reticulation of the gel material. In this protocol, we focus on the preparation of milligram-scale amounts of ~200 nucleotides (nt) RNA molecules that were used in subsequent crystallization experiments.
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Isothermal Titration Calorimetry: Assisted Crystallization of RNA–Ligand Complexesfile of the interaction. Following the ITC experiment, the complex is ready to be concentrated for crystallization trials. This chapter describes a detailed experimental protocol for using ITC as a tool for monitoring RNA/small molecule binding, followed by co-crystallization.
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