Titlebook: Nuclear Reprogramming; Methods and Protocol Kejin Hu Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 iPSC repr

CREST

Generation of Human iPSCs by Reprogramming with the Unmodified Synthetic mRNA, have limitations. Here, I describe a method to reprogram human fibroblasts using a cocktail of mRNAs without any base modification that increases reprogramming efficiency, reduces the RNA-associated toxicity, and yields iPSCs ready for expansion and characterization in as short as 10–14 days.

PANT

Reprogramming of Fibroblasts to Human iPSCs by CRISPR Activators,human fibroblasts using episomal plasmids encoding CRISPRa components. This chapter also provides procedure to assemble guide RNA cassettes and generation of multiplexed guide plasmids for readers who want to design their own guide RNAs.

Intrepid

G-Banded Karyotyping of Human Pluripotent Stem Cell Cultures,normalities. Karyotyping of hPSC cultures can be a challenging undertaking for inexperienced investigators; here, we provide detailed procedures for karyotyping, including sample preparation and analysis, as well as the interpretation of hPSC karyotype results.

cinder

表被动

衰老

Generation of Human Neurons by microRNA-Mediated Direct Conversion of Dermal Fibroblasts,g disorders that affect specific neuronal subtypes. Here, we describe the miR-9/9.-124-based neuronal reprogramming protocols for the generation of several disease-relevant neuronal subtypes: striatal medium spiny neurons, cortical neurons, and spinal cord motor neurons.

backdrop

Efflorescent

羽饰

Kejin Huor special purposes. There are a President, two Vice-Presidents and a ministry, divided into six departments—of Foreign Relations and Worship; Finance; Government and Public Works; Justice and Industry; War and Colonisation; and Education and Agriculture.

先驱

his mountainous district and the similar district of the Yemen, Arabia is a barren country consisting of vast tracts of steppe-desert, sand-waste and mountainous wilderness. It is a country of insignificant rainfall (the Yemen and Oman excepted) ; here and there, scattered oases, or oasis-groups, are formed.