Titlebook: Nuclear Reprogramming; Methods and Protocol Kejin Hu Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 iPSC repr

躺下残杀

Reprogramming of Fibroblasts to Human iPSCs by CRISPR Activators,ctors, human fibroblasts can be reprogrammed into induced pluripotent stem cells (iPSCs). Here, we describe a method for the derivation of iPSCs from human fibroblasts using episomal plasmids encoding CRISPRa components. This chapter also provides procedure to assemble guide RNA cassettes and genera

anagen

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Preparation of Mouse Embryonic Fibroblasts as Feeders for iPSC Generation and Maintenance, proliferation of human pluripotent stem cells (hPSCs). Here, we describe the necessary steps to derive, expand, harvest, inactivate, plate, and use MEFs as feeders for iPSC generation and maintenance.

conifer

Evaluating Reprogramming Efficiency and Pluripotency of the Established Human iPSCS by Pluripotencyf ethical restrictions. One common and initial approach to prove the pluripotency of an established human iPSC line is to demonstrate expression of a set of established surface and intracellular pluripotency markers. This chapter provides procedures of immunocytochemistry of the established HiPSC li

acquisition

G-Banded Karyotyping of Human Pluripotent Stem Cell Cultures,hat regular testing of genetic integrity be conducted. G-banded karyotyping is a widely used genetic assay that is capable of detecting chromosomal abnormalities. Karyotyping of hPSC cultures can be a challenging undertaking for inexperienced investigators; here, we provide detailed procedures for k

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Become Competent in Generating RNA-Seq Heat Maps in One Day for Novices Without Prior R Experience,gical scientists are afraid to prepare heat maps independently because R is a programming platform. Here, using RNA-seq data for 16 differentially expressed genes in . pathway between embryonic stem cells and fibroblasts, I share a tutorial for novices without any prior R experience to master the sk

Morphine

COLON

https://doi.org/10.1007/978-1-0716-1084-8iPSC reprogramming; cardiac progenitors; fibroblasts; episomal vectors; retroviral vectors

预感

978-1-0716-1086-2Springer Science+Business Media, LLC, part of Springer Nature 2021

摊位

Nuclear Reprogramming978-1-0716-1084-8Series ISSN 1064-3745 Series E-ISSN 1940-6029