Titlebook: Noncanonical Amino Acids; Methods and Protocol Edward A. Lemke Book 2018 Springer Science+Business Media, LLC 2018 E. coli.Drosophila.Xenop

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Artificial Division of Codon Boxes for Expansion of the Amino Acid Repertoire of Ribosomal Polypeptiged with nonproteinogenic amino acids by means of flexizymes, the nonproteinogenic and proteinogenic aminoacyl-tRNAs can decode the NNC and NNG codons in the same family codon box independently. In this protocol, we describe expression of model peptides, including a macrocyclic peptide containing th

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壕沟

Genetic Incorporation of Unnatural Amino Acids into Proteins of Interest in , ATCC 15439enables use of other aaRS or aaRS/tRNA pairs for the incorporation of other UAAs; and the vector backbone used allows the system to be transferred to diverse . species via both protoplast transformation and conjugation.

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Expression and Purification of Site-Specifically Lysine-Acetylated and Natively-Folded Proteins for uding X-ray crystallography. This protocol describes the expression and purification of site-specifically lysine-acetylated proteins in . using the genetic-code expansion concept (GCEC) and subsequent steps to assess the successful incorporation of AcK by immunoblotting and mass-spectrometry.

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harmakologischen Institutionen. Im Taschenbuch von Schwabe/Scholz finden Sie hilfreiche Informationen für eine rationale und kostenbewusste Arzneitherapie..978-3-540-26502-3Series ISSN 0937-7433 Series E-ISSN 2512-5214

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