Titlebook: Noncanonical Amino Acids; Methods and Protocol Edward A. Lemke Book 2018 Springer Science+Business Media, LLC 2018 E. coli.Drosophila.Xenop

拿着锡

书目名称Noncanonical Amino Acids影响因子(影响力)




书目名称Noncanonical Amino Acids影响因子(影响力)学科排名




书目名称Noncanonical Amino Acids网络公开度




书目名称Noncanonical Amino Acids网络公开度学科排名




书目名称Noncanonical Amino Acids被引频次




书目名称Noncanonical Amino Acids被引频次学科排名




书目名称Noncanonical Amino Acids年度引用




书目名称Noncanonical Amino Acids年度引用学科排名




书目名称Noncanonical Amino Acids读者反馈




书目名称Noncanonical Amino Acids读者反馈学科排名

mortuary

Cell-Free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Usiminoacyl-tRNA synthetase (aaRS) pairs from ., we have developed CFPS methods for the highly productive and efficient multiple incorporation of ncAAs. In this chapter, we describe our methods for the preparation of the S30 and the orthogonal tRNA. and PylRS pair, and two CFPS protocols for ncAA incorporation.

RENAL

monogamy

Site-Specific Incorporation of Sulfotyrosine Using an Expanded Genetic Codeas noncanonical amino acids (ncAAs) encoded by an expanded genetic code. Here, we describe methods for the co-translational incorporation of one or multiple sulfotyrosines into proteins using standard or genomically recoded . stains, thereby achieving the direct expression of site-specifically tyrosine sulfated proteins in vivo.

谎言

Trapping Chromatin Interacting Proteins with Genetically Encoded, UV-Activatable Crosslinkers In Vivll cycle in synchronized yeast populations. This method thereby provides unprecedented insights into the molecular events controlling chromatin reorganization in mitosis. As similar tools are available for other organisms, it should be possible to derive similar strategies for these and for other synchronizable processes.

确保

SPASM

BULLY

Residue-Specific Incorporation of Noncanonical Amino Acids for Protein Engineering handles (azides and alkynes) and hydrophobic fluorinated side chains. Here, we describe the efficient residue-specific replacement of methionine by azidonorleucine in an engineered green fluorescent protein using a bacterial expression system to introduce a single reactive site for the strain-promoted azide-alkyne cycloaddition.

syncope

Parallel