Titlebook: Noncanonical Amino Acids; Methods and Protocol Edward A. Lemke Book 2018 Springer Science+Business Media, LLC 2018 E. coli.Drosophila.Xenop

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Generation of Stable Amber Suppression Cell Linesediated transposition. A general protocol for the generation of stable amber suppression cell lines is described here. Using the modular plasmid system, homogenous and highly efficient amber suppression in a wide range of cell lines can be achieved.

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978-1-4939-8525-8Springer Science+Business Media, LLC 2018

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Methods in Molecular Biologyhttp://image.papertrans.cn/n/image/667175.jpg

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Leveraging Formylglycine-Generating Enzyme for Production of Site-Specifically Modified Bioconjugatemylglycine-generating enzyme (FGE) is one enzyme that can be exploited in this fashion. FGE binds its consensus sequence (CXPXR, known as the “aldehyde-tag”) and converts the cysteine to a formylglycine (fGly). fGly-containing proteins contain a bioorthogonal aldehyde on their surface that can be mo