Titlebook: Noncanonical Amino Acids; Methods and Protocol Edward A. Lemke Book 2018 Springer Science+Business Media, LLC 2018 E. coli.Drosophila.Xenop

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Artificial Division of Codon Boxes for Expansion of the Amino Acid Repertoire of Ribosomal Polypepticodon boxes consisting of synonymous codons that redundantly code for the same amino acid. Here, we describe the protocol of a recently published method to artificially divide such family codon boxes and encode multiple nonproteinogenic amino acids in addition to the 20 proteinogenic ones in a repro

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Tub-Tag Labeling; Chemoenzymatic Incorporation of Unnatural Amino Acidsrporates noncanonical tyrosine derivatives to the terminal carboxylic acid of proteins containing a 14-amino acid recognition sequence called Tub-tag. The tyrosine derivative carries a unique chemical reporter allowing for a subsequent bioorthogonal modification of proteins with a great variety of p

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Genetic Code Expansion in Enteric Bacterial Pathogensies into proteins of interest in bacteria, yeast, mammalian cells, and even animals. This technique allows precise labeling as well as manipulation of a given protein to dissect its physiological and/or pathological roles under living conditions. Here, we demonstrate the extension of a recently emer

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Residue-Specific Incorporation of Noncanonical Amino Acids for Protein Engineeringcantly broadened the scope of protein engineering allowing introduction of amino acids with non-native functionalities, such as bioorthogonal reactive handles (azides and alkynes) and hydrophobic fluorinated side chains. Here, we describe the efficient residue-specific replacement of methionine by a

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