Titlebook: Non-Natural Nucleic Acids; Methods and Protocol Nathaniel Shank Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 201

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Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-M commonly incorporated into the DNA for subsequent modification by click reaction. However, it is partially oxidized during deprotection during solid-phase synthesis, resulting in a ketone that is no longer accessible for click modification. To enable the high-fidelity solid-phase synthesis of EdU-c

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Site-Specific Labeling of DNA via PCR with an Expanded Genetic Alphabet,fication of DNA with the four-letter genetic alphabet. Here, we describe PCR amplification with a six-letter alphabet that includes the two natural dA-dT and dG-dC base pairs and an unnatural base pair (UBP) formed between the synthetic nucleotides d. and d. or d. or analogs of these synthetic nucle

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Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis,alth. This chapter describes the application of an artificial nucleoside/nucleotide system that functions as a biochemical probe to quantify TLS activity under in vitro and in vivo conditions. For in vitro studies, the artificial nucleotide, 3-ethynyl-5-nitroindolyl-2′-deoxyriboside triphosphate (3-

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Determining Steady-State Kinetics of DNA Polymerase Nucleotide Incorporation,n. Monitoring kinetic aspects of this catalytic process provides mechanistic information regarding polymerase-mediated DNA synthesis and the influences of nucleobase structure. For example, a range of polymerases have different capacities to synthesize DNA depending on the structure of the inserted

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