Titlebook: Non-Natural Nucleic Acids; Methods and Protocol Nathaniel Shank Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 201

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1064-3745 expertsThis volume provides relevant synthetic strategies, incorporation, and applications of non-natural nucleic acids. Chapters detail monomer synthesis, oligomer synthesis/construction, and applications allowing researchers to explore and determine which methodology or methodologies are relevant

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Synthesis of Nucleobase-Functionalized Morpholino Monomers,genations of the nucleobases in suitable position and subsequent Pd-catalyzed Sonogashira and Suzuki reactions. The sequential synthetic steps are described in detail in this chapter, and the examples are shown in tables.

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Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-Montaining DNA, this protocol describes a procedure to perform the click reaction on the solid phase before deprotection. Afterwards, the DNA can be deprotected and purified according to standard procedures, and the full modification of EdU with the azide of choice can be analyzed by HPLC and HPLC/MS.

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Book 2019ligomer synthesis/construction, and applications allowing researchers to explore and determine which methodology or methodologies are relevant to their needs.  Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, li

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,2′-,,4′-,-Ethyleneoxy-Bridged 2′-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesiites into oligonucleotides, (3) UV-melting experiments of the duplexes of the modified oligonucleotides and complementary RNA, and (4) nuclease degradation experiments of the modified oligonucleotides.

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