Titlebook: Next Generation Culture Platforms for Reliable In Vitro Models; Methods and Protocol Tiziana A.L. Brevini,Alireza Fazeli,Kursad Turksen Boo

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Preparation of Biological Scaffolds and Primary Intestinal Epithelial Cells to Efficiently 3D Modele the opportunity of studying complex biological systems in vitro, thereby reducing the need for animal experiments. Artificial intestine models are at the core of Fish-AI, an EU FET-Open research project dedicated to the development of a 3D in vitro platform that is intended to enable the aquacultu

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Book 2021ratory that mimic cell guidance conditions as they occur .in vivo.. The protocols collected describe powerful strategies to exploit chemical cues involved in cell differentiation processes. Special emphasis is given to the use of methods for purification and characterization of exosomes and other se

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A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-like Cells,ne morphogenetic protein 4 (BMP4) with inhibitors of the Activin/Nodal/ERK signaling pathways, to drive erased fibroblasts into the trophoblastic differentiation. This innovative method uses very easily accessible cells to derive trophoblast-like cells and it can be useful to study embryo implantation disorders related to aging.

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Isolation of Extracellular Vesicles (EVs) Using Benchtop Size Exclusion Chromatography (SEC) Columnication as it recovers a sufficient amount of EVs while retaining their functionality. Here, we describe a method of purification of EVs from bovine follicular fluid samples using benchtop SEC columns. In conclusion, the EV purification method should be chosen based on the downstream applications, as every method poses its own limitations.