书目名称 | Next Generation Culture Platforms for Reliable In Vitro Models |
副标题 | Methods and Protocol |
编辑 | Tiziana A.L. Brevini,Alireza Fazeli,Kursad Turksen |
视频video | |
概述 | Includes cutting-edge techniques.Provides step-by-step details essential for reproducible results.Contains key implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | This detailed book collects original protocols aimed at encouraging and stimulating the scientific community to design and produce models for the laboratory that mimic cell guidance conditions as they occur .in vivo.. The protocols collected describe powerful strategies to exploit chemical cues involved in cell differentiation processes. Special emphasis is given to the use of methods for purification and characterization of exosomes and other secreted vesicles, as well as micro and non-coding RNAs, that have been demonstrated to control the tuning of the .in vivo. micro and macro environment in order to ensure the optimal soluble environment .in vitro.. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Next Generation Culture Platforms for Reliable In Vitro Models: Methods and Protocols. serves as an ideal guide for researchers working toward developing these vital testing models for preclinical studies.. |
出版日期 | Book 2021 |
关键词 | Cell culture; 3D culture; Exosomes; Organ development; Drug testing; Functional scaffolds; Nanoparticles |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-0716-1246-0 |
isbn_softcover | 978-1-0716-1248-4 |
isbn_ebook | 978-1-0716-1246-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |