Titlebook: Next Generation Culture Platforms for Reliable In Vitro Models; Methods and Protocol Tiziana A.L. Brevini,Alireza Fazeli,Kursad Turksen Boo

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Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissuesaffolds to promote tissue development is the hallmark of the tissue engineering. Among the different types of scaffolds (derived from either natural or synthetic polymers) used in the field, the use of decellularized tissues/organs is specifically attractive. The decellularization process involves t

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Creation of a Bioengineered Ovary: Isolation of Female Germline Stem Cells for the Repopulation of e represented by the development of a bioprosthetic ovary, obtained through the combination of tissue engineering and regenerative medicine..We here describe the two main steps required for bioengineering the ovary and for its ex vivo functional reassembling. The first step aims at producing a 3D bi

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A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-like Cells,of the placenta and supports the fetus during intrauterine life. Our understanding of these events is limited, particularly in human, because of ethical and legal restrictions and availability of adequate in vitro models would be very advantageous. Here we describe a method that converts human fibro

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Method to Disassemble Spheroids into Core and Rim for Downstream Applications Such as Flow Cytometr adherent cells in cell biology are tailored to this format of cell culturing. Limitations and disadvantages of monolayer cultures, however, have resulted in the ongoing development of advanced cell culturing techniques. One such technique is culturing cells as multicellular spheroids, that had been

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Isolation of Extracellular Vesicles (EVs) Using Benchtop Size Exclusion Chromatography (SEC) Columnells into the extracellular space. The population of EVs being heterogeneous poses a challenge in their efficient separation. Several methods have been employed for EV isolation. However, there is no single conventional method that could recover a high amount of EVs while retaining their purity and

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Measurement of the Size and Concentration and Zeta Potential of Extracellular Vesicles Using Nanopa potential as biomarkers of disease. Nanoparticle tracking analysis (NTA) is a commonly used technique to measure the size and concentration of nanoparticles, such as EVs. Here, we present two protocols for the analysis of size profile concentration, and zeta potential (ZP) of well-characterized EVs

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Isolation, Characterization, and MicroRNA Analysis of Extracellular Vesicles from Bovine Oviduct ancell type and tissue, and are involved in physiological and pathological processes. In recent years, EVs have been identified in reproductive tissues, such as oviduct and uterus, and have been shown to be related to several events important for reproductive success. The understanding of their functi