Titlebook: Neurotrophic Factors; Methods and Protocol Stephen D. Skaper Book 2018Latest edition Springer Science+Business Media, LLC 2018 Neurobiologi

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Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from the Cortex, Spinal Cordthe cortex and spinal cord of neonatal rat and mouse. The procedure is based on enzymatic digestion of the tissue, followed by the culture of a mixed glial cell population which is then utilized as the starting point for the isolation, via differential attachment, of the different cell types.

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Central Nervous System Neuron-Glia co-Culture Models and Application to Neuroprotective Agents,paration of cultures consisting of rat embryonic cortical neurons grown in the presence of cortical microglia, in which the glia are present in physical contact with the neurons or separated by a semipermeable membrane barrier. An example of how such systems can be used to evaluate potential neuropr

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Preparation of Adult Rat Sensory Neuron Cultures and Their Application to Growth Cone Turning Assayted axons in neuromas, injured nerve trunks, or nerves with attempted repair diminish the success of regeneration. The behavior of adult rodent peripheral sensory neurons in vitro, in turn, is helpful in predicting axonal behavior in vivo. Here, we describe the adaptation of embryonic neuron growth

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Sensory Neurons from Tau Transgenic Mice and Their Utility in Drug Screening,n in Alzheimer disease as neurofibrillary tangles (NFTs). Many transgenic mouse models of tauopathies have been generated, but it has been difficult to demonstrate disease in primary brain neurons from these mice because neurons need to be harvested within a few days of birth and tau fails to produc

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Culture of Rat Mesencephalic Dopaminergic Neurons and Application to Neurotoxic and Neuroprotectiveneurons are widely used as a source of dopaminergic neurons for the study of mechanisms implicated in dopaminergic cell death and for the evaluation of potential dopaminergic neuroprotective agents, including neurotrophic factors. This chapter presents a detailed protocol for the preparation of rat

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Astrocyte/Microglia Cocultures as a Model to Study Neuroinflammation,his chapter describes a model which allows one to assess the individual and combined contributions of astrocytes and microglia in response to a pro-inflammatory stimulus, with emphasis on ionotropic purinergic receptors.

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Oligodendrocyte Progenitor Cell Cultures: A Model to Screen Neurotrophic Compounds for Myelin Repaiation for the evaluation of agents with potential trophic activity, that is, which are capable of promoting maturation under such conditions. As an example we have chosen a co-ultramicronized N-palmitoylethanolamine/luteolin composite, a formulation described in the literature as possessing anti-inf

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Cell Enumeration Assays: Application of the MTT and Sulforhodamine B Assays to Lipopolysaccharide-Sic and neuropathic pain. This chapter compares two established cell enumeration assays, namely, the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and the protein-binding sulforhodamine B assay for microglia as a function of culture condition and activation state. T