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Titlebook: Neurotrophic Factors; Methods and Protocol Stephen D. Skaper Book 2018Latest edition Springer Science+Business Media, LLC 2018 Neurobiologi

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书目名称Neurotrophic Factors
副标题Methods and Protocol
编辑Stephen D. Skaper
视频video
概述Includes cutting-edge methods and protocols for the study of neurotrophic factors.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the
丛书名称Methods in Molecular Biology
图书封面Titlebook: Neurotrophic Factors; Methods and Protocol Stephen D. Skaper Book 2018Latest edition Springer Science+Business Media, LLC 2018 Neurobiologi
描述This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, s
出版日期Book 2018Latest edition
关键词Neurobiological research; Animal models; Cell vitality; Neurons and glia; Neurotrophin receptors; Nervous
版次2
doihttps://doi.org/10.1007/978-1-4939-7571-6
isbn_softcover978-1-4939-8524-1
isbn_ebook978-1-4939-7571-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2018
The information of publication is updating

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Preparation of Adult Rat Sensory Neuron Cultures and Their Application to Growth Cone Turning Assay molecule, particularly if it alters growth cones through intra-axonal translation. Similarly, to understand the trajectory of an axon, it must be in a growth mode, such as that associated with preconditioning from previous injury.
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Sensory Neurons from Tau Transgenic Mice and Their Utility in Drug Screening,imilar time course to that found in central nervous system neurons in mice. Thus, NFTs are well-developed around 5 months of age. Because DRG neurons can be cultured from adult mice for months, they have proven to be an excellent model for studying how tau pathology develops and for screening compou
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Methods for Quantitative Analysis of Axonal Cargo Transport,g axons. Then, the procedure to extract relevant parameters from active, diffusive, and confined motion is provided. These properties will allow a better comprehension of the nature and characteristics of cargo motion in living cells, therefore contributing to understanding the consequences of trans
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edrigen Scheitelüberdeckungen tritt jedoch ein Knicken der Auskleidung bei gleichzeitigem Grundbruch im Erdkörper ein. Es zeigt sich, daß dabei die Frage, ob der Außendruck durch den Erdkörper oder als hydraulischer Außendruck zur Wirkung kommt, für das Tragverhalten der Auskleidung von wesentlicher
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Stephen D. Skaper safety. The value of R. was obtained as 0.9983 in both the training and testing phases. The lower values of RMSE (training = 0.0184 and testing = 0.0181) in both phases justify the generalization capability of the model. The finding of this research concludes that the developed model can be used as
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