书目名称 | Neurotrophic Factors | 副标题 | Methods and Protocol | 编辑 | Stephen D. Skaper | 视频video | | 概述 | Includes cutting-edge methods and protocols for the study of neurotrophic factors.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, s | 出版日期 | Book 2018Latest edition | 关键词 | Neurobiological research; Animal models; Cell vitality; Neurons and glia; Neurotrophin receptors; Nervous | 版次 | 2 | doi | https://doi.org/10.1007/978-1-4939-7571-6 | isbn_softcover | 978-1-4939-8524-1 | isbn_ebook | 978-1-4939-7571-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC 2018 |
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