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Titlebook: Neurotransmitter Methods; Richard C. Rayne Book 1997 Humana Press 1997

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发表于 2025-3-21 17:56:03 | 显示全部楼层 |阅读模式
书目名称Neurotransmitter Methods
编辑Richard C. Rayne
视频video
丛书名称Methods in Molecular Biology
图书封面Titlebook: Neurotransmitter Methods;  Richard C. Rayne Book 1997 Humana Press 1997
描述Neurotransmitter Methods is intended as a bench-side companion for researchers who seek to identify, locaHze, or measure neurotransmitters and/or to identify sites of neurotransmitter action. Each method is detailed in a user-friendly "recipe" format and the protocols are accompanied by extensive notes to high­ light and explain crucial steps. Approaches utilizing an incredibly diverse array of modem techniques are presented: methods including HPLC, histochemistry, immunocytochemistry, in situ hybridization, mass spectrometry, microdialysis, and electrochemistry all make at least one appearance. In addition, protocols for associated methodologies, including the production of brain slices, dissociated neurons, synaptosomes/synaptoneurosomes, and neuronal plasma membranes are presented. Methods applicable to most of the recognized chemical types of neu­ rotransmitter are to be found and, although you may find absent any mention of your favorite neurotransmitter, many of the protocols are sufficiently general to be adapted to alternative uses. So, how does this book contribute usefiilly to the horde of methods volumes stampeding across our bookshelves? 1 hope that the strength oiNeuro
出版日期Book 1997
版次1
doihttps://doi.org/10.1385/0896033945
isbn_softcover978-1-61737-023-6
isbn_ebook978-1-59259-558-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 1997
The information of publication is updating

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Alexandra I. M. Breukel,Elly Besselsen,Wim E. J. M. Ghijsen
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David G. Watson,Richard A. Baines,John M. Midgley,Jonathan P. Bacon
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Preparation of Brain Slices,udied can be located, identified, and accessed easily. Use of the brain slice has greatly increased our knowledge of the mammalian central nervous system (CNS). This technique is continuously improving and will remain valuable for a long time.
发表于 2025-3-22 17:51:33 | 显示全部楼层
Preparation of Organotypic Hippocampal Slice Cultures Using the Membrane Filter Method, access to the slice. Most studies now employ variations of the membrane filter method (.). Brain slices are placed on a porous membrane filter that provides a suitable substratum and allows a thin film of media to cover the surface of the slice by capillary action (.). The membrane inserts are plac
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Synaptosomes,ion, synaptosomes maintain a normal membrane potential (which is regulated by a Na./K.-ATPase) and express functional uptake carriers and ion-channels in their plasma-membranes. On application of diverse depolarizing stimuli (e.g., potassium, veratridine, and 4-aminopyridine), Ca. enters synaptosome
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Synaptoneurosomes,perties and a maintained electrochemical gradient (.,.). Since its first description, this preparation has been used for the investigation of numerous phenomena, including neurotransmitter release (.), inositol phospholipid turnover (.,.), CAMP accumulation (.), as well as in functional studies of v
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Detection of Neuropeptides by Immunocytochemistry, relied on ICC, for example: Different neuropeptides have very specific distributions in small populations of neurons (.–.), neuropeptides are commonly colocalized with low-mol-wt neurotransmitters or other neuropeptides (.), the chemical diversity of neurons is far greater than previously suspected
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