Titlebook: Neurotransmitter Methods; Richard C. Rayne Book 1997 Humana Press 1997

Gentry

cGMP-Immunocytochemistry,th serotonim (.–.) and also with small peptides (.). Because the covalent modification of the hapten may be part of the antigenic determinant, it is a prerequisite that antisera are raised against conjugates prepared using a fixative that is also suitable for tissue fixation (.–.).

排出

Microwave Antigen Retrieval in Formaldehyde-Fixed Human Brain Tissue,ormalin fixation, however, the loss of antigenicity is neither complete nor irreversible. Indeed, formaldehydefixed tissue has been successfully used for the detection of many different tissue antigens, such as neurotransmitters (.), neuropeptides (.), cell surface markers (.), and enzymes (.). The

描绘

Book 1997lthough you may find absent any mention of your favorite neurotransmitter, many of the protocols are sufficiently general to be adapted to alternative uses. So, how does this book contribute usefiilly to the horde of methods volumes stampeding across our bookshelves? 1 hope that the strength oiNeuro

Mere仅仅

Neurotransmitter Methods978-1-59259-558-7Series ISSN 1064-3745 Series E-ISSN 1940-6029

出血

Methods in Molecular Biologyhttp://image.papertrans.cn/n/image/664573.jpg

感激小女

雪白

Neuropeptide Expression Patterns as Determined by Matrix-Assisted Laser Desorption Ionization Mass tage of these methods, however, is that they give limited clues to the structural identities of processed and biologically active peptides contained in the cells. Moreover, these techniques do not reveal previously undescribed cotransmitters.

AVANT

The Preparation and Use of Brain Slices and Dissociated Neurons for Patch-Clamp Studies of Neurotraevelopment of the patch-clamp technique has opened up the possibility of direct access to the intracellular environment via internal patch pipet solutions. In combination, these approaches have enabled detailed investigations of neuronal membrane properties, the cellular actions of neurotransmitters, and synaptic mechanisms.

周兴旺

极大痛苦

Microbore Liquid Chromatography Analysis of Monoamine Transmitters,ional LC: The sample injected on to a microbore column is diluted by about 20-fold less than when injected on to a “normal” bore (e.g., 4.6 mm id) column. In this way, the compounds of interest become easier to detect. Microbore LC offers several other advantages: