Titlebook: Neural Stem Cells; Methods and Protocol Marcel M. Daadi Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 neural

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Differentiation of Neural Stem Cells Derived from Induced Pluripotent Stem Cells into Dopaminergic nderlies a wide range of disorders from motor control deficiencies, such as Parkinson’s disease (PD), to psychiatric disorders, such as alcoholism, drug addictions, bipolar disorders, schizophrenia and depression. Neural stem cell-based technology has potential to play an important role in developin

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Midbrain Dopaminergic Neurons Differentiated from Human-Induced Pluripotent Stem Cells,fferentiation, which are eventually modified according to the respective laboratory. In many cases, protocols are not fully described, failing to provide essential tips for researchers starting in the field. Considering that commercial kits produce low mDAN percentages (20–50%), we chose to follow a

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Generating Neural Stem Cells from iPSCs with Dopaminergic Neurons Reporter Gene,ine hydroxylase (TH) is the rate-limiting enzyme for the biosynthesis of the dopamine neurotransmitter, and thus it has been used as a reliable marker for dopaminergic neurons in vitro and in vivo. Herein we describe a method for making iPSC lines with TH-green fluorescent protein reporter gene usin

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Single-Cell Library Preparation of iPSC-Derived Neural Stem Cells,ighly variable genes. Thus, with this technology it is possible to identify relevant pathways involved in development or in disease progression. Herein, we describe a protocol to capture and process single-cell transcriptomes that will be used for RNA sequencing. This chapter discusses the use of th

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Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approacements for differentiation in a systematic way, we present here methodology for an iterative approach using combinations of small molecules and biological factors. The factors are used in a cyclical process in which the best combination of factors and concentrations is selected in one round of testi

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