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Titlebook: Neural Stem Cells; Methods and Protocol Marcel M. Daadi Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 neural

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发表于 2025-3-21 18:08:13 | 显示全部楼层 |阅读模式
书目名称Neural Stem Cells
副标题Methods and Protocol
编辑Marcel M. Daadi
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Neural Stem Cells; Methods and Protocol Marcel M. Daadi Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 neural
描述.This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurological disorders. Chapters guide the reader through methods and protocols on definition and isolation of neural stem cells, characterization and differentiation, standards for defining cell populations, and .In vivo. approaches in animal models.  Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Neural Stem Cells: Methods and Protocols .aims to ensure successful results in the further study of this vital field..
出版日期Book 2019
关键词neural stem cell; stable cellular composition; tissue reprogramming; neural progenitor cells; human plur
版次1
doihttps://doi.org/10.1007/978-1-4939-9007-8
isbn_ebook978-1-4939-9007-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
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Springer Science+Business Media, LLC, part of Springer Nature 2019
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Neural Stem Cells978-1-4939-9007-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
发表于 2025-3-22 05:05:48 | 显示全部楼层
Generating Neural Stem Cells from iPSCs with Dopaminergic Neurons Reporter Gene,ine hydroxylase (TH) is the rate-limiting enzyme for the biosynthesis of the dopamine neurotransmitter, and thus it has been used as a reliable marker for dopaminergic neurons in vitro and in vivo. Herein we describe a method for making iPSC lines with TH-green fluorescent protein reporter gene using CRISPR/Cas9 technique.
发表于 2025-3-22 10:39:10 | 显示全部楼层
Bioinformatics Analysis of Single-Cell RNA-Seq Raw Data from iPSC-Derived Neural Stem Cells,rting with raw sequencing data, we describe how to quality check samples, to create an index from a reference genome, to align the sequences to an index, and to quantify transcript abundances. The curated data sets will enable differential expression analysis, population analysis, and pathway analysis.
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Methods in Molecular Biologyhttp://image.papertrans.cn/n/image/663742.jpg
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