Titlebook: Neisseria gonorrhoeae; Methods and Protocol Myron Christodoulides Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2

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Antimicrobial Resistance in , and Treatment of Gonorrhea, × 1 plus azithromycin 1–2 g × 1) is currently recommended in many countries. These dual therapies have high cure rates, have likely been involved in decreasing the level of cephalosporin resistance internationally, and inhibit the spread of AMR gonococcal strains. However, ceftriaxone-resistant str

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Preparation of Lipooligosaccharide (LOS) from ,r role in pathogenesis by inducing host inflammatory responses and also enabling evasion of host innate immunity through sialylation. Epitopes within LOS are also potential vaccine candidates. In this chapter, we describe a general method based on the Westphal hot phenol extraction process to purify

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Isolation of Naturally Released Gonococcal Outer Membrane Vesicles as Vaccine Antigens,active anti-GC vaccine would best address this global public health threat. Polyantigenic outer membrane vesicles (OMVs) derived from GC can overcome the challenges posed by GC’s high rate of phase and antigen variation. In fact, GC OMVs have already shown promise as a vaccine antigen; however, all

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Strategies for Global RNA Sequencing of the Human Pathogen ,xpression levels for all genes of a particular species or collection of species under a given condition, including genes that are not yet known to exist. While transcriptomics can be a powerful tool to better understand bacterial regulatory responses to specific host environments, the experimental a

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