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Titlebook: Neisseria gonorrhoeae; Methods and Protocol Myron Christodoulides Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2

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Phenotypic MicroArray Screening of , in Chemically Defined Liquid Medium,ein function. Nearly 2000 conditions can be screened relatively quickly either to search for phenotypes associated with the loss of a protein or to understand metabolic differences between closely related bacterial isolates. The fastidious organism . presents an experimental challenge for phenotypic
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Generation of Metal-Depleted Conditions for In Vitro Growth of ,nd replication depend upon successful metal acquisition, these high-affinity uptake systems are important virulence factors. Expression of metal acquisition systems is tightly controlled and preferentially expressed under low-metal conditions. Therefore, in order to optimally produce these transport
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Complement-Dependent Serum Bactericidal Assays for ,important role in host defenses against . infections. Complement-dependent bactericidal activity of antibodies (either natural antibodies or those elicited by immunization) is a well-established correlate of protection against meningococcal infections. Although correlates of protection against gonoc
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Basic Methods for Examining , Interactions with Host Cells In Vitro,i can infect the anorectal, pharyngeal, and gingival mucosae and epithelial cells of the conjunctiva. More rarely, the organism can disseminate through the bloodstream, which can involve interactions with other host cell types, including blood vessel endothelial cells and innate immune cells such as
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Use of Human Monocyte-Derived Macrophages to Study , Infection,gens and macrophages leads to a macrophage response that includes cytokine production, antigen processing and presentation in the context of MHC molecules, expression of T cell costimulatory molecules and recruitment of innate defense effectors, which results in clearance of infection. However, . ca
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How to Identify Exposed Women Who Are Infected with ,parison of women who did, or did not, become infected during exposure, which identifies those women who are highly likely to be infected before culture and/or NAAT results are available. The algorithm provides an efficient way to conduct interventional trials in women without the problem of recall a
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