Titlebook: Natural Killer Cells; Methods and Protocol Srinivas S. Somanchi Book 2016 Springer Science+Business Media New York 2016 cytotoxicity.genoty

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978-1-4939-8116-8Springer Science+Business Media New York 2016

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https://doi.org/10.1007/978-1-4939-3684-7cytotoxicity; genotyping single nucleotide polymorphisms; chemeric antigen receptors; retroviral vector

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Tracking Effector and Memory NK Cells During MCMV Infection,ansion of Ly49H. NK cells peaks at approximately 1 week post-infection, and is then followed by a distinct contraction phase that ultimately leaves a small but long-lived pool of MCMV-experienced Ly49H. NK cells that are capable of mediating enhanced memory-like responses during subsequent encounter

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Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer Cellconjugated to monoclonal antibodies to evaluate up to 42 parameters simultaneously on individual cells with minimal overlap between channels. The platform can be customized for analysis of both phenotypic and functional markers. Here, we will describe methods to stain, collect, and analyze intracell

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Assessment of NK Cell Metabolism, others have begun to investigate the metabolic requirements for NK cell differentiation and activation. Here, we describe approaches for the in vitro assessment of NK cell metabolism. We present methods for using inhibitors to alter cellular metabolism, measurement of intracellular ATP in NK cells,

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Measurement of Average Telomere Length in Ex Vivo Expanded Natural Killer Cells by Fluorescence In through cytokine secretion and target cell lysis. NK cells function without any need for prior antigen exposure. Thus, more recently NK cells are considered a promising source of lymphocytes for adoptive tumor therapy. However, because NK cells represent only a small lymphocyte fraction, expand poor