| 书目名称 | Natural Killer Cells | | 副标题 | Methods and Protocol | | 编辑 | Srinivas S. Somanchi | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This volume contains collection of Natural Killer Cell methodologies relevant for both basic and translational research. These methodologies present new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells metabolism on its function, identifying complexity of NK cell subsets through mass cytometry, and determining the emergence of memory NK cells in murine model of MCMV infection. Methods that study NK cell migration and cytotoxicity through endpoint analysis or live single cell imaging are also discussed. Chapters also describe methods pertaining to translational application of NK cells, such as .ex vivo. expansion of NK cells on K562 cell lines genetically modified to express either membrane bound IL-15 or membrane bound IL-21, large scale NK cell culture, current techniques for engineering NK cells to express chimeric antigen receptors or chemokine receptors using retroviral vectors,electroporation of mRNA, and the natural phenomenon of trogocytosis. Written in the highly successful .Methods in Molecular Biology .series format, these chapters include introductions to their respective topics, lists of the necessary materials and rea | | 出版日期 | Book 2016 | | 关键词 | cytotoxicity; genotyping single nucleotide polymorphisms; chemeric antigen receptors; retroviral vector | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-3684-7 | | isbn_softcover | 978-1-4939-8116-8 | | isbn_ebook | 978-1-4939-3684-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 2016 |
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发表于 2025-3-21 16:29:48
