Titlebook: Natural Killer Cells; Methods and Protocol Srinivas S. Somanchi Book 2016 Springer Science+Business Media New York 2016 cytotoxicity.genoty

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In Vitro Assessment of Human Natural Killer Cell Migration and Invasion,r invasion assays represent a reductionist system that allows for easy manipulation using various extracellular matrix (ECM) components addressing migratory functions or invasion into/through a three-dimensional matrix where migration and invasion inhibitors as well as stimulators can be added. Pres

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Microfluidic-Based Live-Cell Analysis of NK Cell Migration In Vitro,ments. NK cells express specific chemokine and non-chemokine receptors to aid preferential migrations or localizations in tissues. Good understanding of how NK-cell migratory properties are regulated in physiological and pathological microenvironments will provide further insights into the developme

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Microwell-Based Live Cell Imaging of NK Cell Dynamics to Assess Heterogeneity in Motility and Cytotmetry, providing static snapshots. These approaches have certain drawbacks, not enabling dynamic observations of single cells over extended periods of time. One of the primary limitations of single cell imaging has been throughput; it has been challenging to collect data for many cells due to their

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Assessment of Natural Killer Cell Cytotoxicity Using Image Cytometry Method, cells is the direct cytolysis of their targets. Hence analyzing the cytotoxic potential of these lymphocytes is fundamental to understanding their biology and their clinical impact. We have previously shown that release-based cytotoxicity assays, such as calcein release assay, could potentially und

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Analysis of Intracellular Ca2+ Mobilization in Human NK Cell Subsets by Flow Cytometry, cytokine production. Upon cellular activation, intracellular mobilization of Ca. ions initially involves depletion of endoplasmic reticulum stores, leading to subsequent Ca. influx through specific plasma membrane Ca. release activated Ca. channels. Multiple probes and assays for detecting intracel

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Using NK Cell Lipid Raft Fractionation to Understand the Role of Lipid Rafts in NK Cell Receptor Sid not be confused with lipid rafts, DRMs are a valuable starting point for the study of these membrane domains and the interactions of proteins with rafts..Here we describe the isolation of DRMs by ultracentrifugation on a sucrose gradient, a method we have used to study the role of lipid rafts in N

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The Planar Lipid Bilayer System Serves as a Reductionist Approach for Studying NK Cell Immunologica or a tumor cell). The formation of the IS is crucial for cell-mediated immunity, and as such, an understanding of both the composition of the IS and the factors that drive its formation are essential for understanding how and when NK cells eliminate susceptible target cells. The supported lipid bil

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Ex Vivo Expansion of Human NK Cells Using K562 Engineered to Express Membrane Bound IL21,rder to evaluate the therapeutic efficacy of NK cell adoptive immunotherapy in preclinical models with a potential for clinical translation, there is a need for a reliable platform for ex vivo expansion of NK cells. Numerous methods are reported in literature using cytokines and feeder cells to acti