Titlebook: Nanoscale Imaging; Methods and Protocol Yuri L. Lyubchenko Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 org

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Yuri L. LyubchenkoIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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978-1-4939-9336-9Springer Science+Business Media, LLC, part of Springer Nature 2018

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High-Resolution Atomic Force Microscopy Imaging of Nucleic Acidso its close relative double-stranded RNA. Here, we provide a comprehensive description of the methods employed and the steps required to image the helical periodicity of these two nucleic acids with the sample immersed in a buffer solution.

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Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy physiological function of the molecule. In this chapter, we describe protocols for preparation of substrates that have been used for HS-AFM and then introduce observation examples on dynamic processes of biological molecules.

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High-Resolution and High-Speed Atomic Force Microscope Imagingt only a few labs in the world had access to (including ours) to an established commercialized technology that is present in tens of labs around the world. In this protocol chapter we share with the readers our practical know-how on high resolution HS-AFM imaging.

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High-Speed Atomic Force Microscopy of Individual Amyloidogenic Protein Assembliesctural dynamics of relevant biomolecules for various biological phenomena. Here, we describe the methods for HS-AFM observation and the analysis of the structural dynamics of individual amyloidogenic protein assemblies using amyloid β 1–42 as an example.