Titlebook: Nanoscale Imaging; Methods and Protocol Yuri L. Lyubchenko Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 org

绝缘

PACT

Supported Lipid Bilayers for Atomic Force Microscopy Studieslipid bilayer (SLB), which itself is a traditional model for cellular membranes. Success in these studies is based on the availability of a stable SLB for the required observation period, which can extend several hours. The application of AFM requires that the SLB have a smooth morphology, thus enab

Asseverate

Graduated

Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy conditions. One of the key factors leading to successful HS-AFM observations is the selection of an appropriate substrate depending on molecules to be observed. For the HS-AFM imaging, a target molecule must be absorbed on a substrate by controlling its orientation without impairing the dynamics or

AGONY

membrane

CHURL

Chauvinistic

Assembly of Centromere Chromatin for Characterization by High-Speed Time-Lapse Atomic Force Microscoging in ambient conditions can provide details of the conformational states and interactions of a population of molecules which is well complemented by single-molecule imaging of the systems dynamics using time-lapse AFM imaging, in which images are capture at rates of 10–15 frames per second in an

Blatant

Fierce

Probing RNA–Protein Interactions with Single-Molecule Pull-Down Assayswith high spatiotemporal resolution. However, it remains challenging to obtain functional eukaryotic protein complexes and cost-effective fluorescently labeled RNAs to study their interactions at the single-molecule level. Here, we describe protocols combining single-molecule fluorescence with vario