Titlebook: NMDA Receptors; Methods and Protocol Nail Burnashev,Pierre Szepetowski Book 2017 Springer Science+Business Media LLC 2017 NMDARs.plasticity

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Gene Targeted Mice with Conditional Knock-In (-Out) of NMDAR Mutationslysis of nonsense or missense mutations that were identified in human patients, single nucleotide mutations have to be introduced into the germ line of mice (Burnashev and Szepetowski, Curr Opin Pharmacol 20:73–82, 2015; Endele et al., Nat Genet 42:1021–1026, 2010). This can be done with very high p

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GluNs Detection and Functions in Microglial Cellsated, (2) their cellular localization, (3) that the GluN are functional, and (4) the role of the functional GluN. The complete demonstration is performed by using mRNA detection technics, western blots, immunofluorescence, electrophysiology, calcium imaging, morphology studies, multiplex immunoassay

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NMDA Receptor Activity in Circulating Red Blood Cells: Methods of Detections and, by doing that, controls red cell volume, membrane stability, and O. carrying capacity. Detection of the NMDA receptor activity in red blood cells is challenging as the number of its copies is low and shows substantial cell-to-cell heterogeneity. Receptor abundance is reliably assessed using t

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NMDA Receptors as Voltage Sensorsssociated with a number of artifacts. Cell-attached recordings of the currents through NMDA receptor channels enable noninvasive measurements of the neuronal membrane potential. This approach overcomes the problem of a leak conductance introduced during intracellular sharp electrode recordings and w

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