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Titlebook: NMDA Receptors; Methods and Protocol Nail Burnashev,Pierre Szepetowski Book 2017 Springer Science+Business Media LLC 2017 NMDARs.plasticity

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发表于 2025-3-21 16:47:23 | 显示全部楼层 |阅读模式
书目名称NMDA Receptors
副标题Methods and Protocol
编辑Nail Burnashev,Pierre Szepetowski
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: NMDA Receptors; Methods and Protocol Nail Burnashev,Pierre Szepetowski Book 2017 Springer Science+Business Media LLC 2017 NMDARs.plasticity
描述.This volume explores the latest techniques used by researchers to help them better understand the NMDAR structure-function relations, principles, and rules that govern how NMDARs operate in brain processing. The volume includes a detailed introductory chapter describing the field and is divided into three parts. Topics covered in the volume include: quantification of NMDAR subunit genes expression by qRT-PCR; detection of NMDARs antibodies in encephalitis; recombinant channels in host cells using a fast agonist application system; GluNs detection and functions in microglial cells; and NMDARs as voltage sensors. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and practical, .NMDA Receptors: Methods and Protocols .is a valuable resource that discusses the diversity of the currently-used methods, the importance of NMDARs and their complexity, and the progress that has already been made. This book will be of interest to scien
出版日期Book 2017
关键词NMDARs; plasticity; epilepsy; CNS; encephalitis; high-resolution live imaging
版次1
doihttps://doi.org/10.1007/978-1-4939-7321-7
isbn_softcover978-1-4939-8452-7
isbn_ebook978-1-4939-7321-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2017
The information of publication is updating

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发表于 2025-3-21 20:59:10 | 显示全部楼层
GluN2B Subunit Labeling with Fluorescent Probes and High-Resolution Live Imagingeptor by conjugation of fluorescein to an ifenprodil-based ligand, (2) an in vitro functional assay by calcium imaging for GluN2B binding and inhibition evaluation of the probe, and (3) the labeling and confocal imaging of GluN2B in DS-red labeled living cortical neurons.
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Design of Light-Sensitive NMDARs by Genetically Encoded Photo-Cross-Linkers synaptic transmission. Through heterologous expression in . oocytes, we have identified light-sensitive NMDARs of GluN2B subtype by using the two-electrode voltage electrophysiology measurement in combination with online-UV application. Immunoblotting analysis has been used to confirm inter-subunit crosslinking.
发表于 2025-3-22 10:13:43 | 显示全部楼层
Analysis of Functional NMDA Receptors in Astrocyteshodology how to study the physiology of NMDA receptors selectively on astrocytes will be described in detail. Astrocytes are the main class of neuroglia that control transmitter and ion homeostasis, which link cerebral blood flow and neuronal energy demands, but also affect synaptic transmission directly.
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Functional Analysis of Recombinant Channels in Host Cells Using a Fast Agonist Application Systemproperties of the channels. Currently, one of the most well-developed techniques suitable for addressing these issues is use of the fast agonist application system for rapid activation of ligand gated ion-channels (Colquhoun et al., J Physiol 458:261–287, 1992; Jonas and Sakmann, J Physiol 455:143–171, 1992).
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Electrophysiological Investigation of NMDA Current Properties in Brain Slicesgical tools, and the analysis of the difference in temporal parameters between the AMPA and NMDA receptors. It allows pinpointing of the basic functional properties of NMDARs that are specific to identified brain regions, neurons, and synapses of wild-type or genetically manipulated mice.
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