Titlebook: NF-κB Transcription Factors; Methods and Protocol Guido Franzoso,Francesca Zazzeroni Book 2021 Springer Science+Business Media, LLC, part o

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Biochemical Methods to Analyze the Subcellular Localization of NF-κB Proteins Using Cell Fractionatiing their contents, thus allowing an enrichment of the protein of interest. Using different concentrations of sucrose or detergent buffer formulations in combination with centrifugations, the cell fractions are separated based on their density and size.

Carcinoma

Immunohistochemical Analysis of Expression, Phosphorylation, and Nuclear Translocation of NF-κB Proter molecules. This technique allows a snapshot of the structure of tissue and determines the cellular and subcellular localization of a target antigen. This chapter describes how to identify and localize NF-κB proteins in human tissue using immunohistochemical staining on formalin-fixed paraffin-emb

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Molecular and Biochemical Approaches to Study the Evolution of NF-κB Signaling in Basal Metazoansexample, in members of the phyla Cnidaria (e.g., sea anemones, corals, hydra, jellyfish) and Porifera (sponges), and in several single-celled protists (e.g., ., some choanoflagellates). Therefore, methods are required to study the function of NF-κB and its pathway members in early branching organism

Aerophagia

Methods for Modulating the Pathway of NF-κB Using Short Hairpin RNA (ShRNA)lack of targeting drugs, or because of the risk of wider off-target effects. Here we describe the design and use of short hairpin RNA (ShRNA) and lentiviral vectors as an efficient technique for silencing NF-kappaB (NF-κB) pathway in cultured cells. This method can be used also in hard to transfect

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经典

Methods to Study CARD11-BCL10-MALT1 Dependent Canonical NF-κB Activation in Jurkat T Cellsor (TCR)/CD28 co-stimulation. The critical function of the key regulators identified in Jurkat T cells has subsequently been verified in primary murine and human T cells. CRISPR/Cas9-mediated genomic editing techniques in combination with viral reconstitution are powerful tools that now enable the i

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Hyperalgesia