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Titlebook: NF-κB Transcription Factors; Methods and Protocol Guido Franzoso,Francesca Zazzeroni Book 2021 Springer Science+Business Media, LLC, part o

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书目名称NF-κB Transcription Factors
副标题Methods and Protocol
编辑Guido Franzoso,Francesca Zazzeroni
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: NF-κB Transcription Factors; Methods and Protocol Guido Franzoso,Francesca Zazzeroni Book 2021 Springer Science+Business Media, LLC, part o
描述This detailed book serves as a systematic examination of the analytical methods to study the transcription factor NF-κB in physiology and disease. It provides an up-to-date guidebook to navigate both conventional and highly specialized methods to detect and analyze the different signaling pathways of NF-κB activation and contextualize them within organismal physiology and disease pathogenesis, using genetic and biochemical techniques and some of the most advanced computational and systems biology methods. Additionally, the volume includes several examples of approaches utilized by leading experts in the NF-κB field to analyze and modulate NF-κB signaling in specific physiological and disease contexts, along with some of the most promising approaches to pharmacologically target the NF-κB pathway in human disease. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .NF-κB Transcription Factors: Methods and Protocols.
出版日期Book 2021
关键词Nuclear factor kappa-B; Dysregulation in human diseases; Signaling pathways; NF-κB activation; Pharmacol
版次1
doihttps://doi.org/10.1007/978-1-0716-1669-7
isbn_softcover978-1-0716-1671-0
isbn_ebook978-1-0716-1669-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

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Biochemical Methods to Analyze the Subcellular Localization of NF-κB Proteins Using Cell Fractionatiing their contents, thus allowing an enrichment of the protein of interest. Using different concentrations of sucrose or detergent buffer formulations in combination with centrifugations, the cell fractions are separated based on their density and size.
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Methods for Modulating the Pathway of NF-κB Using Short Hairpin RNA (ShRNA)lack of targeting drugs, or because of the risk of wider off-target effects. Here we describe the design and use of short hairpin RNA (ShRNA) and lentiviral vectors as an efficient technique for silencing NF-kappaB (NF-κB) pathway in cultured cells. This method can be used also in hard to transfect primary cell cultures.
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Measuring NF-κB Phosphorylation and Acetylationcontrol the transcriptional outcomes of this important transcription factor. These modifications work independently, sequentially or in combination to modulate the diverse biological functions of NF-κB in cancer and inflammatory response. Here, we describe some experimental methods to detect the in
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