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Titlebook: NF-kappa B; Methods and Protocol Michael J. May Book 2015 Springer Science+Business Media New York 2015 DELFIA.Electrophoretic Mobility Shi

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Stable Reconstitution of IKK-Deficient Mouse Embryonic Fibroblastseature of this technique is the ability to create cell lines that stably express the protein of interest and can be cultured long term. Here we describe a retroviral transduction procedure for mouse embryonic fibroblasts (MEFs) that uses Platinum-E cells to rapidly package high-titer, helper-free re
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Visualizing TCR-Induced POLKADOTS Formation and NF-κB Activation in the D10 T-Cell Clone and Mouse Ps, stimulates the T-cell receptor (TCR), leading to activation of the transcription factor, nuclear factor kappa B (NF-κB). The subsequent NF-κB-dependent gene expression program drives expansion and effector differentiation of antigen-specific T cells, leading to the adaptive anti-pathogen immune r
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Analysis of Epidermal Growth Factor-Induced NF-κB Signalingom our lab indicates that CARMA3, similar to the function of CARMA1 in mediating antigen receptor-mediated NF-κB activation, plays an essential role in mediating EGFR-induced NF-κB activation. However, the mechanism on how EGFR induces NF-κB activation is not clearly understood. In this chapter, we
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