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Titlebook: NF-kappa B; Methods and Protocol Michael J. May Book 2015 Springer Science+Business Media New York 2015 DELFIA.Electrophoretic Mobility Shi

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发表于 2025-3-21 19:30:34 | 显示全部楼层 |阅读模式
书目名称NF-kappa B
副标题Methods and Protocol
编辑Michael J. May
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: NF-kappa B; Methods and Protocol Michael J. May Book 2015 Springer Science+Business Media New York 2015 DELFIA.Electrophoretic Mobility Shi
描述.Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-κB activation, regulation, and function. .NF-kappa B: Methods and Protocols .guides readers through standard approaches to detect NF-κB pathway activation, detection and analysis of NF-κB signaling, and methods to study the control of NF-κB signaling. As a volume in the highly successful .Methods in Molecular Biology. series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls..Comprehensive and authoritative, .NF-Kappa B: Methods and Protocols .provides a timely and invaluable resource for researchers seeking to perform experiments aimed at understanding the role of NF-κB signaling in health and disease..
出版日期Book 2015
关键词DELFIA; Electrophoretic Mobility Shift; Epidermal Growth Factor (EGF); IKK activity; NF-κB pathway activ
版次1
doihttps://doi.org/10.1007/978-1-4939-2422-6
isbn_softcover978-1-4939-4811-6
isbn_ebook978-1-4939-2422-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
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Detection of IκB Degradation Dynamics and IκB-α UbiquitinationB pathway activation and can serve as a quantitative parameter to assess pathway activation. In this article, we present a detailed protocol for the quantification of in vivo ubiquitination and turnover of IκB-α in response to a variety of cellular stimuli.
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1064-3745 ation advice from the experts.Includes supplementary materia.Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-κB activation, regulation, and function. .NF-kappa B: Methods and Protocols .guides readers through standard approaches to detec
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Methods to Assess the Activation of the Alternative (Noncanonical) NF-κB Pathway by Non-death TNF Reis chapter straightforward methods to monitor the activation of the alternative NF-κB pathway. These methods uncover cytosolic and nuclear biochemical modifications of key proteins of the alternative NF-κB pathway required prior to the transcription of NF-κB target genes.
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Book 2015n. .NF-kappa B: Methods and Protocols .guides readers through standard approaches to detect NF-κB pathway activation, detection and analysis of NF-κB signaling, and methods to study the control of NF-κB signaling. As a volume in the highly successful .Methods in Molecular Biology. series, chapters c
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