Titlebook: Microinjection; Methods and Protocol Chengyu Liu,Yubin Du Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Gene

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Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation,oinjection has been the technology of choice, electroporation has also emerged and been proven to be effective delivering CRISPR-Cas9 reagents into the mouse zygote. Here, we describe the experimental protocol employing electroporation to deliver CRISPR-Cas9 reagents into mouse embryos and derive gene-edited mutant mice.

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Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer,ce, including derivation and inactivation of MEF feeder cells, culturing and handling of mouse ES cells, collection and microinjection of blastocysts, and finally implantation of injected blastocysts into the uteri of pseudopregnant surrogate mothers.

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Creating Knockin Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombina as in classic gene targeting. As the result, knockin alleles across many target loci are created in mouse embryonic stem cells and readily transmitted through germline. The knockin alleles created in ES cells can also serve as valuable tools for in vitro stem cell differentiation.

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Wenhao Xu undertook in response to their key challenges. Regular online reflections about their transition into rural teaching were collected through ., a digital tool (see Gibson A, Willis J, Morrison C, Crosswell L, Not losing the plot: creating, collecting and curating qualitative data through a web-based

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Xin-an Pu,Anthony P. Young,H. Michael Kubischational relations, famine, and debt.Based on untapped origin.This book analyses the evolution of the city of Rome, in particular, papal Rome, from the plague of 1656 until 1870 when it became the capital of the Kingdom of Italy. The authors explore papal Rome as a resilient city that had to cope wit

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Yubin Du,Wen Xie,Fan Zhang,Uimook Choi,Chengyu Liu,Colin L. Sweeneyor challenges. In recent years, with the emergence of sustainability issues, particularly the climate issue, resilience has been associated with countries’ sustainability. Furthermore, many resilience attributes seem to be founded and reinforced through intellectual capital components. Nowadays, res

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Ye Liu,Yubin Du,Wen Xie,Fan Zhang,Douglas Forrest,Chengyu Liurception, particularly in terms of timeframe between parties in land use conflicts..A brief review is made of conflicts reported from Mediterranean climate ecosystems which indicate that there is no universal pattern and hence no universal prescription for resolving conflicts in land use. This appea