书目名称 | Microinjection | 副标题 | Methods and Protocol | 编辑 | Chengyu Liu,Yubin Du | 视频video | | 概述 | Includes cutting-edge techniques for the study of microinjection and its purposes.Focuses on mouse models for half of the book, with the other half centered on a wide variety of other models.Contains | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Microinjection: Methods and Protocols. serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations.. | 出版日期 | Book 2019 | 关键词 | Gene editing; Animal model generation; CRISPR; TALEN; Embryo micromanipulations; Genome engineering | 版次 | 1 | doi | https://doi.org/10.1007/978-1-4939-8831-0 | isbn_softcover | 978-1-4939-9398-7 | isbn_ebook | 978-1-4939-8831-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2019 |
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