Titlebook: Microbial Transposon Mutagenesis; Protocols and Applic Steven C. Ricke,Si Hong Park,Morgan L. Davis Book 2019 Springer Science+Business Med

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Construction of a Sequence-Defined Transposon Mutant Library in ,tants, each containing a single transposon insertion mutation within nearly all of the nonessential genes within the genome, is a rapid and reliable way to enhance the study of gene function. In this chapter, we describe the process to generate a sequence-defined transposon mutant library in . utilizing the mariner-based . transposon.

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dry-eye

Protocols of Conjugative Plasmid Transfer in ,: Plate, Broth, and Filter Mating Approachesdonor and the recipient. Self-conjugative plasmids harbor . genes which facilitate plasmid transfer from donor to recipient bacterial strain. Here we describe different methods of conjugative plasmid transfers via conjugation.

Meditative

affinity

Transposon-Aided Capture of Antibiotic Resistance Plasmids from Complex Sampleshe removal of contaminating chromosomal DNA. Here we describe isolation of bacterial cells from a complex sample, DNA extraction, transposon-aided capture of plasmids in the sample, and analysis of the captured plasmids.

intricacy

Efficient Gene Deletion Method for , heat-sensitive origin of replication, four unique restriction sites (.I, .I, .I, and .I), and erythromycin resistance gene. We demonstrated that pHoss1 is very efficient for introducing mutations into different . strains. In this chapter, we include a brief description of pHoss1 and the method used for gene deletion in . using pHoss1.

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Transposon Mutagenesis in , Speciesmid pMN100 into ., carry out transposon mutagenesis, and determine the chromosomal location of inserted transposons. It is our prospect that the protocols can be used as guidelines for transposon mutagenesis in . as well as other species of streptococci.

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