书目名称 | Microbial Transposon Mutagenesis |
副标题 | Protocols and Applic |
编辑 | Steven C. Ricke,Si Hong Park,Morgan L. Davis |
视频video | |
概述 | Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as .Salmonella. to .Bifidobacterium., a microorganism considered beneficial to humans and animals. The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Microbial. .Transposon Mutagenesis: Protocols and Applications. serves as a valuable reference for scientists seeking to apply transposon mutagenesis to microbial genetic analyses and functionality.. |
出版日期 | Book 2019 |
关键词 | Phenotypic characteristics; Transposon mutant libraries; Whole genome sequencing; Salmonella; Microorgan |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-9570-7 |
isbn_softcover | 978-1-4939-9572-1 |
isbn_ebook | 978-1-4939-9570-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media, LLC, part of Springer Nature 2019 |