Titlebook: Microbial Steroids; Methods and Protocol José-Luis Barredo,Ignacio Herráiz Book 2017 Springer Science+Business Media LLC 2017 Terpenoid lip

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Steroid Bioconversions,ming strains in combination with novel approaches in the enzymatic and whole-cell biocatalysis provide a platform for highly effective and selective biotransformations..Here, we briefly review the current state and prospects in the field of microbial bioconversions with special attention to the appl

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Stigmasterol Removal by an Aerobic Treatment System, 29% to 37% at a low stigmasterol load rate (0.2 mg/L/day). On the other hand, the stigmasterol is removed between 65% and 87% by an AS under a hydraulic retention time of 3 h. Moreover, a 100% of stigmasterol can be removed by the MBBR when the hydraulic retention time is 2 days.

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Bioconversion of Phytosterols into Androstenedione by ,,on, some steroids, notably ADD, may be toxic to cells..Two main strategies have been employed to overcome this challenge: the use of two-phase systems, and the addition of chemically modified cyclodextrins. The latter method is used here..Defined cultivation and bioconversion media for both shake fl

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,Obtaining of 11α-Hydroxyandrost-4-ene-3,17-dione from Natural Sterols,ains, to yield about 70% (mol/mol). At the second step, AD is almost fully (98%) hydroxylated at the position 11α with . VKM F-830, or other suitable organisms, in the same bioreactor. At the average, 30% (w/w) of the high-purity crystalline 11α-HAD can be obtained..The method can be exploited for p

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Steroid 11-Alpha-Hydroxylation by the Fungi , and ,,m to introduce them in other microorganisms, such as ., used in the industry to split the side chain of phytosterols, and thus creating recombinant microorganisms able to generate useful steroids from cheap precursors in just one-step fermentation.

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1064-3745 lls.. . Comprehensive and thorough, .Microbial Steroids: Methods and Protocols .is a valuable resource for laboratory and industrial professionals. It is also useful for graduate students studying biotechnology, microbiology, genetics, and molecular biology..978-1-4939-8410-7978-1-4939-7183-1Series ISSN 1064-3745 Series E-ISSN 1940-6029

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,β-Sitosterol Bioconversion to Androstenedione in Microtiter Plates,. Here, we describe the production of 4-androstene-3,17-dione (androstenedione; AD), a key pharmaceutical steroid intermediate, by . sp. NRRL B-3805 via the selective cleavage of the side-chain of β-sitosterol using 24-well microtiter plates.

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Book 2017ly reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. . Comprehensive and thorough, .Microbial Steroids: Methods and Protocols .is a valuable resource for laboratory and industrial professionals. It is also useful for graduate students studying biotechnology, microbiology, genetics, and molecular biology..

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Genome Sequencing of Steroid-Producing Bacteria with Illumina Technology,d following data bioinformatics treatment. Software for genome assembly and completion are listed that assemble, map, annotate, visualize, edit and allow doing other manipulations with genomic sequences. The whole genomes sequencing of the steroid-producing Actinobacteria using these protocols is exemplified.

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