Titlebook: Microbial Metabolic Engineering; Methods and Protocol Christine Nicole S. Santos,Parayil Kumaran Ajikuma Book 2019 Springer Science+Busines

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Metabolic In Silico Network Expansions to Predict and Exploit Enzyme Promiscuity,pacities often go unnoticed in the enzyme’s native context but can become apparent in engineered metabolism when the enzyme is exposed to novel substrates or high concentrations of pathway intermediates. This chapter describes how to use metabolic in silico network expansion (MINE) databases to pred

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Interlocking

Chemically Inducible Chromosomal Evolution (CIChE) for Multicopy Metabolic Pathway Engineering,, flanking an antibiotic selection marker and a gene of interest with identical regions of homology permits gene duplication via . mediated homologous recombination. A strong selective pressure for gene duplication can be applied by increasing antibiotic concentration, and in a week’s time one can c

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Bacterial Expression of Membrane-Associated Cytochrome P450s and Their Activity Assay in Nanodiscs,nction studies. Recently, there have been several instances of expressing eukaryotic membrane bound CYPs in bacteria after making various modifications to both the N-terminus membrane binding domains of the protein and to noncontiguous F-G membrane binding loop that is also implicated in substrate b

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Checks and Balances with Use of the Keio Collection for Phenotype Testing,ce cassette in place of a single nonessential gene. This mutant library has proven to be incredibly useful in the fields of bacteriology, chemical genomics, biotechnology, and systems biology, which is evidenced by the greater than 3800 citations that the article describing its construction has garn

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Genetic Tools for Streamlined and Accelerated Pathway Engineering in ,hough it has a long history of industrial applications, the number of genetic tools available to effectively and efficiently engineer . still falls behind the vast number of tools available for common organisms such as . and .. In this protocol, we have developed a complete and versatile genetic too