Titlebook: Microbial Metabolic Engineering; Methods and Protocol Christine Nicole S. Santos,Parayil Kumaran Ajikuma Book 2019 Springer Science+Busines

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书目名称Microbial Metabolic Engineering影响因子(影响力)




书目名称Microbial Metabolic Engineering影响因子(影响力)学科排名




书目名称Microbial Metabolic Engineering网络公开度




书目名称Microbial Metabolic Engineering网络公开度学科排名




书目名称Microbial Metabolic Engineering被引频次




书目名称Microbial Metabolic Engineering被引频次学科排名




书目名称Microbial Metabolic Engineering年度引用




书目名称Microbial Metabolic Engineering年度引用学科排名




书目名称Microbial Metabolic Engineering读者反馈




书目名称Microbial Metabolic Engineering读者反馈学科排名

Blood-Clot

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Antisense RNA Elements for Downregulating Expression,yl-carrier-protein] transacylase as an example to describe the asRNA design with reliable and controllable interference efficiency. Real-time PCR and fluorescence assay methods are introduced to detect the interference efficiency at RNA level and protein level, respectively.

爆米花

Chemically Inducible Chromosomal Evolution (CIChE) for Multicopy Metabolic Pathway Engineering, recombination. A strong selective pressure for gene duplication can be applied by increasing antibiotic concentration, and in a week’s time one can create a set of strains with a wide range of cassette copy numbers (upward of 20×), which can be made stable by deletion of .. Herein, we describe a generalized workflow for this methodology.

anthropologist

Genetic Engineering of Cyanobacteria: Design, Implementation, and Characterization of Recombinant , detailed including methods to test promoter strength using the green fluorescent protein reporter. Furthermore, a method for markerless transformation of chromosomal DNA is presented. Sufficient details are provided to enable application by the novice investigator.

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1064-3745 ation advice from the expertsThis volume covers a wide array of topics that will aid researchers in the task of engineering complex biological systems. This book is divided into three parts: Part One discusses the discovery and identification of relevant biosynthetic pathways for engineering; Part T

CLASP

Assembly of Complex Pathways Using Type IIs Restriction Enzymes,red for the construction strategy. Basic parts that are not yet available need to be made. Multigene constructs are then assembled using a series of one-pot assembly steps with the set of identified parts and vectors.

Defraud

Checks and Balances with Use of the Keio Collection for Phenotype Testing,es that can be employed to ensure robust phenotype assessment of mutations in the library. These include procedures for thorough confirmation of gene deletions by PCR, phage transduction of mutated loci to new host strains, and strategies for genetic complementation.

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