Titlebook: Methods of Protein Separation; Nicholas Catsimpoolas Book 1975 Springer Science+Business Media New York 1975 amino acid.biological.biology

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Isoelectric Focusing in Polyacrylamide Gel,eric macromolecules. IEF is essentially an equilibrium method for segregating amphoteric molecules by electrophoresis in stable pH gradients according to their isoelectric points (p.). In only one or two experiments it is theoretically possible to display all components whose p. values differ by as

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Purification of Chemically Modified Proteins,cture. In recent years, the application of modern knowledge of proteins and the uses of new chemical reagents and more sophisticated analytical techniques have made chemical modifications of proteins a major tool in protein chemistry (Hirs and Timasheff, 1972.; Means and Feeney, 1971). But, even wit

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Sedimentation Equilibrium of Proteins in Density Gradients,ad in 1957 (Meselson .., 1957). The method provides a thermodynamically rigorous procedure for the determination of the buoyant density, hydration, and solvated molecular weight of polymers having molecular weights larger than about 10,000.

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